A long period grating-based platform for the detection of E. coli proteins

Raquel Barbosa Queirós, Carlos A. J. Gouveia, José R. A. Fernandes, Paulo V. S. Marques, João Paulo da Costa de Noronha, M. Goreti F. Sales

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

A Long Period Grating (LPG)-based platform for the detection of E. coli outer membranes proteins (EcOMPs) is presented. The sensing probe is achieved by the functionalization of a LPG inscribed in a single mode fiber (SMF28) with a DNA-aptamer resulting in a label-free configuration capable of specific recognize EcOMPs in waters. Measurement takes place by tracking the variations induced in the resonance wavelength by the refractive index changes at the fiber surface (≈100 nm/RIU). The sensing head was characterized and tested against EcOMPs and applied to spiked environmental water samples. The sensor displayed logarithmic responses in the range of 0.1 nM to 10 nM EcOMPs and is regenerated (under low pH conditions) and the deviation of the subsequent detection was less than 0.1 %.
Original languageEnglish
Title of host publication8th Iberoamerican Optics Meeting, RIAO 2013 and 11th Latin American Meeting on Optics, Lasers, and Applications, OPTILAS 2013
EditorsM. F. P. C. M. Costa
Place of PublicationBellingham
PublisherSPIE-International Society for Optical Engineering
ISBN (Print)978-0-8194-9601-0
DOIs
Publication statusPublished - 2013
Event8th Iberoamerican Optics Meeting, RIAO 2013 and 11th Latin American Meeting on Optics, Lasers, and Applications, OPTILAS 2013 - Porto, Portugal
Duration: 22 Jul 201326 Jul 2013

Publication series

NameProceedings of SPIE
PublisherSPIE-International Society for Optical Engineering
Volume8785
ISSN (Print)0277-786X

Conference

Conference8th Iberoamerican Optics Meeting, RIAO 2013 and 11th Latin American Meeting on Optics, Lasers, and Applications, OPTILAS 2013
CountryPortugal
CityPorto
Period22/07/1326/07/13

Keywords

  • Aptamer
  • Biosensor
  • E. coli
  • Evanescent field
  • Long Period Gratings

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