A Novel Type of Monoheme Cytochrome c: Biochemical and Structural Characterization at 1.23 angstrom Resolution of Rhodothermus marinus Cytochrome c

Meike Stelter, Ana M. P. Melo, Manuela M. Pereira, Claudio M. Gomes, Gudmundur O. Hreggvidsson, Sigridur Hjorleifsdottir, Ligia M. Saraiva, Miguel Teixeira, Margarida Archer

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

Monoheme cytochromes of the C-type are involved in a large number of electron transfer processes, which play an essential role in multiple pathways, such as respiratory chains, either aerobic or anaerobic, and the photosynthetic electron transport chains. This study reports the biochemical characterization and the crystallographic structure, at 1.23 Å resolution, of a monoheme cytochrome c from the thermohalophilic bacterium Rhodothermus marinus. In addition to an α-helical core folded around the heme, common for this type of cytochrome, the X-ray structure reveals one unusual α-helix and a unique N-terminal extension, which wraps around the back of the molecule. Based on a thorough structural and amino acid sequence comparison, we propose R. marinus cytochrome c as the first characterized member of a new class of C-type cytochromes.
Original languageEnglish
Pages (from-to)11953-11963
Number of pages11
JournalBiochemistry
Volume47
Issue number46
DOIs
Publication statusPublished - 18 Nov 2008

Keywords

  • Electron transport properties
  • Amines
  • Amino acids
  • Organic acids

Cite this