Affinity tags in protein purification and peptide enrichment: An overview

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

The reversible interaction between an affinity ligand and a complementary receptor has been widely explored in purification systems for several biomolecules. The development of tailored affinity ligands highly specific toward particular target biomolecules is one of the options in affinity purification systems. However, both genetic and chemical modifications in proteins and peptides widen the application of affinity ligand-tag receptors pairs toward universal capture and purification strategies. In particular, this chapter will focus on two case studies highly relevant for biotechnology and biomedical areas, namely the affinity tags and receptors employed on the production of recombinant fusion proteins, and the chemical modification of phosphate groups on proteins and peptides and the subsequent specific capture and enrichment, a mandatory step before further proteomic analysis.

Original languageEnglish
Title of host publicationProtein Downstream Processing
EditorsN. E. Labrou
Place of PublicationNew York
PublisherHumana Press Inc.
Pages107-132
Number of pages26
ISBN (Electronic)978-1-62703-977-2
ISBN (Print)978-1-62703-976-5
DOIs
Publication statusPublished - 2021

Publication series

NameMethods in Molecular Biology
PublisherHumana Press Inc.
Volume2178
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Affinity purification
  • Affinity tags
  • Fusion proteins
  • Phosphoproteomics
  • Recombinant proteins

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