Antifungal-resistant Mucorales in different indoor environments

Liliana Aranha Caetano, Tiago Faria, Jan Springer, Juergen Loeffler, Carla Viegas

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)
56 Downloads (Pure)

Abstract

This paper sought to address the prevalence of Mucorales in different indoor environments in Portugal. Environmental samples (183 in total) were collected at dwellings (n = 79) and workplaces (bakeries, swine farms, taxis, waste-sorting plants) (n = 93) by passive sampling using electrostatic dust collector (EDC), air-conditioning filters, litter, and/or raw materials. Samples were inoculated onto non-selective MEA and DG18 media and were screened for antifungal drug-resistance in azole-supplemented agar Sabouraud media. A probe-based Mucorales-specific real-time PCR assay (Muc18S) was used to detect Mucorales in complement to conventional culture-based methods. Mucorales order was found as more prevalent in air-conditioning filters from waste-sorting fork lifters (35.7%). Amongst Mucorales isolates able to grow in azole-supplemented media, 16 isolates of Mucor sp., Rhizopus sp. or Rhizomucor sp. were not susceptible to 1 mg/L voriconazole, and four isolates of Mucor sp. or Rhizopus sp. were not susceptible to 4 mg/L itraconazole. In conclusion, combination of the culture-based and molecular methods proved to be reliable for Mucorales order identification in complex environmental samples.

Original languageEnglish
Pages (from-to)75–83
JournalMycology
Volume10
Issue number2
DOIs
Publication statusPublished - 2019

Keywords

  • antifungal drug-resistance
  • indoor environments
  • Mucorales

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