A preliminary application of diffusion-ordered spectroscopy (DOSY) for separation, identification and characterisation of metabolites of human lung carcinoma cell extracts, exhibiting different levels of multi-drug resistance (MDR) is reported. The results show the presence of at least three lipid components of different sizes in the lipophilic extracts which can not be identified by one- and two-dimensional NMR techniques. A number of metabolites in the hydrophilic extracts of human lung carcinoma cell lines are successfully separated by different diffusivities. The appearance in the DOSY spectra of different metabolites with the same diffusion coefficient is consistent with them being chemically bound together.
|Journal||Bulgarian Chemical Communications|
|Publication status||Published - 1 Jan 2008|