Colorimetric assessment of BCR-ABL1 transcripts in clinical samples via gold nanoprobes

Raquel Vinhas, Claudia Correia, Patricia Ribeiro, Alexandra Lourenco, Aida Botelho de Sousa, Maria Alexandra Núncio de Carvalho Ramos Fernandes, Pedro Miguel Ribeiro Viana Baptista

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Gold nanoparticles functionalized with thiolated oligonucleotides (Au-nanoprobes) have been used in a range of applications for the detection of bioanalytes of interest, from ions to proteins and DNA targets. These detection strategies are based on the unique optical properties of gold nanoparticles, in particular, the intense color that is subject to modulation by modification of the medium dieletric. Au-nanoprobes have been applied for the detection and characterization of specific DNA sequences of interest, namely pathogens and disease biomarkers. Nevertheless, despite its relevance, only a few reports exist on the detection of RNA targets. Among these strategies, the colorimetric detection of DNA has been proven to work for several different targets in controlled samples but demonstration in real clinical bioanalysis has been elusive. Here, we used a colorimetric method based on Au-nanoprobes for the direct detection of the e14a2 BCR-ABL fusion transcript in myeloid leukemia patient samples without the need for retro-transcription. Au-nanoprobes directly assessed total RNA from 38 clinical samples, and results were validated against reverse transcription-nested polymerase chain reaction (RT-nested PCR) and reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The colorimetric Au-nanoprobe assay is a simple yet reliable strategy to scrutinize myeloid leukemia patients at diagnosis and evaluate progression, with obvious advantages in terms of time and cost, particularly in low- to medium-income countries where molecular screening is not routinely feasible.

Original languageEnglish
Pages (from-to)5277-5284
Number of pages8
JournalAnalytical and Bioanalytical Chemistry
Volume408
Issue number19
DOIs
Publication statusPublished - Jul 2016

Keywords

  • Chronic myeloid leukemia
  • Leukemia
  • RNA
  • Gold nanoparticles
  • Colorimetric detection
  • CHRONIC MYELOID-LEUKEMIA
  • CHRONIC MYELOGENOUS LEUKEMIA
  • TYROSINE KINASE INHIBITORS
  • STEM-CELLS
  • ABL
  • MANAGEMENT
  • RECOMMENDATIONS
  • IMATINIB
  • DIAGNOSTICS
  • INTERFERON

Cite this