Effect of the morphogene bolA on the permeability of the Escherichia coli outer membrane

Patrick Freire, Helena L.A. Vieira, Ana R. Furtado, Miguel A. De Pedro, Cecília M. Arraiano

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

Escherichia coli bolA is a morphogene involved in stress response and cell division. Overexpression of bolA induces biofilm formation and affects the levels of carboxypeptidases PBP5, PBP6 and β-lactamase AmpC. In this study, we have shown that changes in the expression of bolA result in alterations in the properties of the outer membrane. The sensitivity to detergents and vancomycin was reduced when bolA was overexpressed and fluorescent probes indicated that different levels of bolA had an effect on outer membrane protein accessibility. Moreover, bolA was shown to be involved in the modulation of the OmpF/OmpC balance.

Original languageEnglish
Pages (from-to)106-111
Number of pages6
JournalFEMS Microbiology Letters
Volume260
Issue number1
DOIs
Publication statusPublished - 1 Jul 2006

Keywords

  • bolA
  • Cell morphology
  • Escherichia coli
  • Outer membrane
  • Permeability

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