Human dendritic cells contain cell surface sialyltransferase activity

M. Guadalupe Cabral, A. Rita Piteira, Zélia Silva, Dário Ligeiro, Reinhard Brossmer, Paula A. Videira

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Human monocyte-derived dendritic cells (mo-DCs) express highly sialylated structures, with recognized but poorly understood function in maturation, immunogenicity and endocytosis capacity. We have previously shown that mo-DCs surface sialylation is changeable upon different stimuli, which led us to hypothesise the existence of cell surface (non-intracellular) sialyltransferases, rapidly restoring or altering mo-DC surface sialylation, thus modulating specific functions.Here, we demonstrate that, in the presence of exogenous CMP-Neu5Ac, mo-DCs incorporate considerable amounts of sialic acids into cell surface, predominantly when mo-DCs were previously desialylated or matured. This is a genuine sialyltransferase activity, confirmed by specific inhibition assays, which is not influenced by secreted enzymes. Functionally, the ecto-sialyltransferase activity causes a significant down-regulation of mo-DCs endocytic capacity, without affecting the maturation state.These findings suggest that ecto-sialyltransferases participate in a dynamic control of mo-DC sialylation, with functional repercussions. This activity is possibly related with specific physiological and pathological conditions, as inflammation and infection, contributing to protection and homeostasis regulation.

Original languageEnglish
Pages (from-to)89-96
Number of pages8
JournalImmunology Letters
Volume131
Issue number1
DOIs
Publication statusPublished - 1 Jun 2010

Keywords

  • Dendritic cell
  • Ecto-sialyltransferase
  • Endocytosis
  • Sialic acid

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