Induced immunity against the mosquito Anopheles stephensi (Diptera: Culicidae): Effects of cell fraction antigens on survival, fecundity, and Plasmodium berghei (Eucoccidiida: Plasmodiidae) transmission

A. Paulo G. Almeida, Peter F. Billingsley

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19 Citations (Scopus)

Abstract

Two subcellular fractions from the midgut of the malaria mosquito Anopheles stephensi (Liston) were used to immunize BALB/c mice. Mice were subsequently infected with the rodent malaria parasite Plasmodium berghei (Vincke & Lips), and the effects of anti-mosquito immunity on mosquito survival and fecundity and on parasite transmission were investigated. Mosquitoes were infected directly from mice (in vivo) or by feeding cultured ookinetes through a membrane (in vitro). Infections were monitored by counting oocysts on the midgut wall. Microvilli extracts induced a strong and partially specific antibody reaction against the midgut, which was manifest as decreased survival in in vivo fed mosquitoes and reduced fecundity in both kinds of feeding. Antisera against microvilli reduced the mean intensity of P. berghei oocysts when fed in vitro, while mosquitoes fed antiserum against basolateral plasma membranes in vivo, showed higher oocyst burdens.

Original languageEnglish
Pages (from-to)207-214
Number of pages8
JournalJournal of Medical Entomology
Volume39
Issue number1
DOIs
Publication statusPublished - Jan 2002

Keywords

  • Anopheles stephensi
  • Anti-mosquito antibodies
  • Midgut microvilli
  • Plasmodium berghei malaria

UN Sustainable Development Goals (SDGs)

  • SDG 3 - Good Health and Well-Being

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