Induced immunity against the mosquito Anopheles stephensi: Reactivity characteristics of immune sera

A. P.G. Almeida, P. F. Billingsley

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

This study shows the progression of immune responses in mice during five sequential immunizations with Anopheles stephensi mosquito extracts, characterized by ELISA, Western blot and immunohistochemistry. When exposed repeatedly to mosquito bites, control mice developed antibodies which reached titres of 1:105, reacted weakly in Western blot analysis and were localized to the salivary glands. Mice immunized with mosquito head plus salivary glands, midgut, ovary, fat body, midgut microvilli (Mv) and midgut basolateral plasma membrane (Blm), showed increased titre with each successive boost. Epitopes were shared between sera or were specific to the immunizing or heterologous extract. Anti-Mv and Blm sera recognized proteins labelled by anti-midgut serum and gave specific reactions with the midgut and head. Cross-reactivity was confirmed immunohistochemically.

Original languageEnglish
Pages (from-to)53-64
Number of pages12
JournalMedical and Veterinary Entomology
Volume13
Issue number1
DOIs
Publication statusPublished - Feb 1999

Keywords

  • Anopheles stephensi
  • Anti-mosquito antibodies
  • Basolateral membrane
  • Immune responses
  • Microvilli
  • Midgut
  • Mosquito
  • Mouse
  • Salivary glands

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