PCR as a rapid and sensitive tool in the diagnosis of human and canine leishmaniasis using Leishmania donovani s.l.: specific kinetoplastid primers

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Abstract

This study was performed in order to test the efficacy of a new polymerase chain reaction (PCR) assay for the diagnosis of both human and canine leishmaniasis caused by Leishmania infantum. The new primers were developed on the basis of a complete DNA sequence of the L. infantum kinetoplast minicircle. Specificity and sensitivity were evaluated by testing bone marrow spots on filter paper and skin biopsy samples, and the PCR results were compared to data from in vitro cultures. Leishmania strains from different foci, as well as other trypanosomatids and opportunistic pathogenic micro-organisms, were also included in this study. The results show that the primers are highly specific, detecting only L. donovani s.l. DNA, and sensitive for the detection of parasite DNA in biological samples from three different geographical regions of Portugal (north, centre and south) and from Brazil.

Original languageEnglish
Pages (from-to)12-17
Number of pages6
JournalTransactions of the Royal Society of Tropical Medicine and Hygiene
Volume98
Issue number1
DOIs
Publication statusPublished - 1 Jan 2004

Keywords

  • Kinetoplastid primers
  • Leishmania donovani s.l.
  • Leishmaniasis
  • Polymerase chain reaction

UN Sustainable Development Goals (SDGs)

  • SDG 3 - Good Health and Well-Being

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