Protection and Reactivation of the [NiFeSe] Hydrogenase from Desulfovibrio vulgaris Hildenborough under Oxidative Conditions

Adrian Ruff, Julian Szczesny, Sónia Zacarias, Inês A.C. Pereira, Nicolas Plumeré, Wolfgang Schuhmann

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

We report on the fabrication of bioanodes for H2 oxidation based on [NiFeSe] hydrogenase. The enzyme was electrically wired by means of a specifically designed low-potential viologen-modified polymer, which delivers benchmark H2 oxidizing currents even under deactivating conditions owing to efficient protection against O2 combined with a viologen-induced reactivation of the O2 inhibited enzyme. Moreover, the viologen-modified polymer allows for electrochemical co-deposition of polymer and biocatalyst and, by this, for control of the film thickness. Protection and reactivation of the enzyme was demonstrated in thick and thin reaction layers.

Original languageEnglish
Pages (from-to)964-968
Number of pages5
JournalACS Energy Letters
Volume2
Issue number5
DOIs
Publication statusPublished - 12 May 2017

Fingerprint Dive into the research topics of 'Protection and Reactivation of the [NiFeSe] Hydrogenase from Desulfovibrio vulgaris Hildenborough under Oxidative Conditions'. Together they form a unique fingerprint.

Cite this