TY - JOUR
T1 - Proteomics for Drug Resistance on the Food Chain? Multidrug-Resistant Escherichia coli Proteomes from Slaughtered Pigs
AU - Ramos, Sónia
AU - Silva, Nuno
AU - Hébraud, Michel
AU - Santos, Hugo M.
AU - Nunes-Miranda, Júlio Dinis
AU - Pinto, Luís
AU - Pereira, Jose E.
AU - Capelo, José Luis
AU - Poeta, Patrícia
AU - Igrejas, Gilberto
N1 - Sem PDF.
The authors gratefully acknowledge the financial support from national funds of Fundacao para a Ciencia e Tecnologia (FCT), cofinanced by POPH QREN Type 4.1-Advanced Training and subsidized by the European Social Fund and National Funds of Ministry of Science and Technology for High Education (MCTES), to Sonia Ramos, J.D., Nunes-Miranda, and Hugo Santos (SFRH/BD/47706/2008, SFRH/BD/80001/2011, SFRH/BPD/73997/2010, respectively) and from "Programa Ciencia 2008,'' co-financed by POPH QREN Type 4.2-Employment Promotion Scientific subsidized by the European Social Fund and MCTES, to N.S. The authors are also grateful to the Research Unit on Applied Molecular Biosciences (UCIBIO), which is financed by national funds from FCT/MEC (UID/Multi/04378/2013) and cofinanced by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728).
PY - 2016/6/1
Y1 - 2016/6/1
N2 - Understanding global drug resistance demands an integrated vision, focusing on both human and veterinary medicine. Omics technologies offer new vistas to decipher mechanisms of drug resistance in the food chain. For example, Escherichia coli resistance to major antibiotics is increasing whereas multidrug resistance (MDR) strains are now commonly found in humans and animals. Little is known about the structural and metabolic changes in the cell that trigger resistance to antimicrobial agents. Proteomics is an emerging field that is used to advance our knowledge in global health and drug resistance in the food chain. In the present proteomic analysis, we offer an overview of the global protein expression of different MDR E. coli strains from fecal samples of pigs slaughtered for human consumption. A full proteomic survey of the drug-resistant strains SU60, SU62, SU76, and SU23, under normal growth conditions, was made by two-dimensional electrophoresis, identifying proteins by MALDI-TOF/MS. The proteomes of these four E. coli strains with different genetic profiles were compared in detail. Identical transport, stress response, or metabolic proteins were discovered in the four strains. Several of the identified proteins are essential in bacterial pathogenesis (GAPDH, LuxS, FKBPs), development of bacterial resistance (Omp's, TolC, GroEL, ClpB, or SOD), and potential antibacterial targets (FBPA, FabB, ACC's, or Fab1). Effective therapies against resistant bacteria are crucial and, to accomplish this, a comprehensive understanding of putative resistance mechanisms is essential. Moving forward, we suggest that multi-omics research will further improve our knowledge about bacterial growth and virulence on the food chain, especially under antibiotic stress.
AB - Understanding global drug resistance demands an integrated vision, focusing on both human and veterinary medicine. Omics technologies offer new vistas to decipher mechanisms of drug resistance in the food chain. For example, Escherichia coli resistance to major antibiotics is increasing whereas multidrug resistance (MDR) strains are now commonly found in humans and animals. Little is known about the structural and metabolic changes in the cell that trigger resistance to antimicrobial agents. Proteomics is an emerging field that is used to advance our knowledge in global health and drug resistance in the food chain. In the present proteomic analysis, we offer an overview of the global protein expression of different MDR E. coli strains from fecal samples of pigs slaughtered for human consumption. A full proteomic survey of the drug-resistant strains SU60, SU62, SU76, and SU23, under normal growth conditions, was made by two-dimensional electrophoresis, identifying proteins by MALDI-TOF/MS. The proteomes of these four E. coli strains with different genetic profiles were compared in detail. Identical transport, stress response, or metabolic proteins were discovered in the four strains. Several of the identified proteins are essential in bacterial pathogenesis (GAPDH, LuxS, FKBPs), development of bacterial resistance (Omp's, TolC, GroEL, ClpB, or SOD), and potential antibacterial targets (FBPA, FabB, ACC's, or Fab1). Effective therapies against resistant bacteria are crucial and, to accomplish this, a comprehensive understanding of putative resistance mechanisms is essential. Moving forward, we suggest that multi-omics research will further improve our knowledge about bacterial growth and virulence on the food chain, especially under antibiotic stress.
KW - OUTER-MEMBRANE PROTEINS
KW - 2-DIMENSIONAL ELECTROPHORESIS
KW - ANTIMICROBIAL RESISTANCE
KW - GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE
KW - FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE
KW - FUNCTIONAL-CHARACTERIZATION
KW - MYCOBACTERIUM-TUBERCULOSIS
KW - STREPTOCOCCUS-PNEUMONIAE
KW - NALIDIXIC-ACID
KW - IDENTIFICATION
UR - http://www.scopus.com/inward/record.url?scp=84975489278&partnerID=8YFLogxK
U2 - 10.1089/omi.2016.0044
DO - 10.1089/omi.2016.0044
M3 - Article
C2 - 27310477
AN - SCOPUS:84975489278
VL - 20
SP - 362
EP - 374
JO - Omics-A Journal Of Integrative Biology
JF - Omics-A Journal Of Integrative Biology
SN - 1536-2310
IS - 6
ER -