The purification, crystallization and identification by X-ray diffraction analysis of a horse kallikrein is reported. The protein was purified from horse seminal plasma. Crystals belong to space group C2 and the structure was solved by the MIRAS method, with two heavy-atom derivatives of mercury and platinum. X-ray diffraction data to 1.42 Å resolution were collected at the ESRF synchrotron-radiation source.
|Number of pages||4|
|Journal||Acta Crystallographica Section D: Biological Crystallography|
|Publication status||Published - 23 Aug 2001|