Purification, crystallization and identification by X-ray analysis of a prostate kallikrein from horse seminal plasma

A. L. Carvalho, J. M. Dias, L. Sanz, A. Romero, J. J. Calvete, M. J. Romão

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

The purification, crystallization and identification by X-ray diffraction analysis of a horse kallikrein is reported. The protein was purified from horse seminal plasma. Crystals belong to space group C2 and the structure was solved by the MIRAS method, with two heavy-atom derivatives of mercury and platinum. X-ray diffraction data to 1.42 Å resolution were collected at the ESRF synchrotron-radiation source.

Original languageEnglish
Pages (from-to)1180-1183
Number of pages4
JournalActa Crystallographica Section D: Biological Crystallography
Volume57
Issue number8
DOIs
Publication statusPublished - 23 Aug 2001

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