RNAi-based glyconanoparticles trigger apoptotic pathways for in vitro and in vivo enhanced cancer-cell killing

João Conde, Furong Tian, Yulan Hernandez, Chenchen Bao, Pedro Miguel Ribeiro Viana Baptista, Daxiang Cui, Tobias Stoeger, Jesus M. de la Fuente

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

Gold glyconanoparticles (GlycoNPs) are full of promise in areas like biomedicine, biotechnology and materials science due to their amazing physical, chemical and biological properties. Here, siRNA GlycoNPs (AuNP@PEG@Glucose@siRNA) in comparison with PEGylated GlycoNPs (AuNP@PEG@Glucose) were applied in vitro to a luciferase-CMT/167 adenocarcinoma cancer cell line and in vivo via intratracheal instillation directly into the lungs of B6 albino mice grafted with luciferase-CMT/167 adenocarcinoma cells. siRNA GlycoNPs but not PEGylated GlycoNPs induced the expression of pro-apoptotic proteins such as Fas/CD95 and caspases 3 and 9 in CMT/167 adenocarcinoma cells in a dose dependent manner, independent of the inflammatory response, evaluated by bronchoalveolar lavage cell counting. Moreover, in vivo pulmonary delivered siRNA GlycoNPs were capable of targeting c-Myc gene expression (a crucial regulator of cell proliferation and apoptosis) via in vivo RNAi in tumour tissue, leading to an similar to 80% reduction in tumour size without associated inflammation.

Original languageEnglish
Pages (from-to)9083-9091
Number of pages9
JournalNanoscale
Volume7
Issue number19
DOIs
Publication statusPublished - 2015

Keywords

  • HUMAN-ENDOTHELIAL CELLS
  • GOLD NANOPARTICLES
  • CARBOHYDRATE INTERACTIONS
  • DNA-DAMAGE
  • C-MYC
  • GLYCOLYSIS
  • EXPOSURE
  • MODELS
  • TOOLS
  • DEATH

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