Bioactivity guided fractionation of Echiochilon fruticosum Desf. ( Boraginaceae) butanolic extract biautography assay against fungi led to the isolation of a new bioactive alkaloid, named saoussanabiloide ( 1). Its structure was established on the basis of spectroscopic measurements, IR, MS and 2D NMR using COSY, HMQC and HMBC experiments. The strongest inhibitory effect of the butanolic extract, from fractions derived from the crude extract and saoussanabiloide ( 1), were observed against Fusarium oxysporum f. sp. niveum.