Serosurvey of Borrelia in dogs, horses, and humans exposed to ticks in a rural settlement of southern Brazil

Denise Nascimento (Denise Amaral Gomes Nascimento), Rafael Vieira (Rafael Felipe da Costa Vieira), Thállitha Vieira (Thállitha Samih Wischral Jayme Vieira), Roberta Toledo (Roberta dos Santos Toledo), Katia Tamekuni, Nelson Santos (Nelson Jessé Rodrigues dos Santos), Daniela Gonçalves (Daniela Dibb Gonçalves), Maria Luisa Vieira, Alexander Biondo (Alexander Welker Biondo), Odilon Vidotto

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Abstract

The aims of the present study were to serosurvey dogs, horses, and humans highly exposed to tick bites for anti-Borrelia burgdorferi s.l. antibodies, identify tick species present, and determine risk factors associated with seropositivity in a rural settlement of Paraná State, southern Brazil. Eighty-seven residents were sampled, along with their 83 dogs and 18 horses, and individual questionnaires were administered. Immunofluorescence antibody test (IFAT) was performed on serum samples and positive samples were subjected to western blot (WB) analysis. Anti-B. burgdorferi antibodies were found in 4/87 (4.6%) humans, 26/83 (31.3%) dogs, and 7/18 (38.9%) horses by IFAT, with 4/4 humans also positive by WB. Ticks identified were mostly from dogs and included 45/67 Rhipicephalus sanguineus, 21/67 Amblyomma ovale, and 1/67 A. cajennense sensu lato. All (34/34) horse ticks were identified as A. cajennense s.l.. No significant association was found when age, gender, or presence of ticks was correlated to seropositivity to Borrelia sp. In conclusion, although anti-Borrelia antibodies have been found in dogs, horses and their owners from the rural settlement, the lack of isolation, molecular characterization, absence of competent vectors and the low specificity of the commercial WB kit used herein may have impaired risk factor analysis.
Original languageEnglish
Pages (from-to)418-422
Number of pages5
JournalBrazilian Journal of Veterinary Parasitology
Volume25
Issue number4
DOIs
Publication statusPublished - Dec 2016

Keywords

  • Immunofluorescence antibody test (IFAT); serology;
  • Lyme disease
  • Tick-borne disease
  • Serology
  • Western blot

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