Structural and functional analysis of the gene cluster encoding carotenoid biosynthesis in Mycobacterium aurum A+

Miguel Viveiros, Philipp Krubasik, Gerhard Sandmann, Mohamed Houssaini-Iraqui

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)

Abstract

The fragment containing the carotenoid gene cluster from Mycobacterium aurum A+, a 3,3'-dihydroxy-isoneriatene and 3-monohydroxy-isoneriatene accumulator, has been sequenced and the exposed eight genes are organised in two operons. The function of three of these genes, a phytoene desaturase (crtI), a phytoene cyclase (crtY) and a β-carotene desaturase (crtU), was demonstrated by complementation of M. aurum carotenoid mutants. The eight genes of the carotenoid cluster are highly homologous to other carotenoid gene clusters and thus this cluster is a candidate for its introduction into mycobacteria as a non-antibiotic reporter gene(s) as well as a source of new regulated promoters. Copyright (C) 2000 Federation of European Microbiological Societies.

Original languageEnglish
Pages (from-to)95-101
Number of pages7
JournalFEMS Microbiology Letters
VolumeVol. 187
Issue numbern.º 1
DOIs
Publication statusPublished - 1 Jun 2000

Keywords

  • Carotenogenic gene cluster expression
  • Isoneriatene synthase
  • Mycobacterium aurum
  • Phytoene cyclase
  • Phytoene desaturase

UN Sustainable Development Goals (SDGs)

  • SDG 3 - Good Health and Well-Being

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