Structural characterization and biological properties of silver(I) tris(pyrazolyl)methane sulfonate

J. Almeida, Catarina Roma-Rodrigues, Abdallah G. Mahmoud, M. Fátima C. Guedes da Silva, Armando J. L. Pombeiro, Luísa M. D. R. S. Martins, Pedro V. Baptista, Alexandra R. Fernandes

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

The water-soluble 1D helical coordination polymer [Ag(Tpms)]n (1) [Tpms = tris(pyrazolyl)methane sulfonate, O3SC(pz)3; pz = pyrazolyl] was synthesized and fully characterized, its single-crystal X-ray diffraction analysis revealing the ligand acting as a bridging chelate N3-donor ligand. The antiproliferative potential of 1 was performed on two human tumour cell lines, A2780 and HCT116, and in normal fibroblasts, with a much higher effect in the former cell line (IC50 of 0.04 μM) as compared to the latter cell line and to normal fibroblasts. Compound 1 does not alter cell cycle progression but interferes with the adherence of A2780 cells triggering cell apoptosis. Apoptosis appears to occur via the extrinsic pathway (no changes in mitochondria membrane potential, reactive oxygen species (ROS) and pro-apoptotic (B-cell lymphoma 2 (BCL-2) associated protein (BAX))/anti-apoptotic (BCL-2) ratio) being this hypothesis also supported by the presence of silver mainly in the supernatants of A2780 cells. Results also indicated that cell death via autophagy was triggered. Proteomic analysis allowed us to confirm that compound 1 is able to induce a stress response in A2780 cells that is related with its antiproliferative activity and the trigger of apoptosis.

Original languageEnglish
Article number110789
JournalJournal of Inorganic Biochemistry
Volume199
DOIs
Publication statusPublished - 1 Oct 2019

Keywords

  • 2-D electrophoresis
  • Antiproliferative activity
  • Cell death
  • Ovarian carcinoma
  • Silver complexes

Fingerprint Dive into the research topics of 'Structural characterization and biological properties of silver(I) tris(pyrazolyl)methane sulfonate'. Together they form a unique fingerprint.

Cite this