Systematics of basidiomycetous yeasts: A comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions

Gloria Scorzetti, J. W. Fell, A. Fonseca, Adele Statzell-Tallman

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350 Citations (Scopus)

Abstract

Basidiomycetous yeasts in the Urediniomycetes and Hymenomycetes were examined by sequence analysis in two ribosomal DNA regions: the D1/D2 variable domains at the 5′ end of the large subunit rRNA gene (D1/D2) and the internal transcribed spacers (ITS) 1 and 2. Four major lineages were recognized in each class: Microbotryum, Sporidiobolus, Erythrobasidium and Agaricostilbum in the Urediniomycetes; Tremellales, Trichosporonales, Filobasidiales and Cystofilobasidiales in the Hymenomycetes. Bootstrap support for many of the clades within those lineages is weak; however, phylogenetic analysis provides a focal point for in-depth study of biological relationships. Combined sequence analysis of the D1/D2 and ITS regions is recommended for species identification, while species definition requires classical biological information such as life cycles and phenotypic characterization.

Original languageEnglish
Pages (from-to)495-517
Number of pages23
JournalFems Yeast Research
Volume2
Issue number4
DOIs
Publication statusPublished - 1 Dec 2002

Keywords

  • Hymenomycete
  • Phylogenetic analysis
  • Urediniomycete
  • Yeast

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