Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 697-717 |
| Number of pages | 21 |
| Journal | Protist |
| Volume | 168 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2017 |
Keywords
- AFM
- cap assembly
- cilia assembly
- re-ciliation.
- Tetrahymena
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Tetrahymena Cilia Cap is Built in a Multi-step Process: A Study by Atomic Force Microscopy. / Seixas, C.; Gonçalves, J.; Melo, L.V.; Soares, H.
In: Protist, Vol. 168, No. 6, 2017, p. 697-717.Research output: Contribution to journal › Article › peer-review
TY - JOUR
T1 - Tetrahymena Cilia Cap is Built in a Multi-step Process: A Study by Atomic Force Microscopy
AU - Seixas, C.
AU - Gonçalves, J.
AU - Melo, L.V.
AU - Soares, H.
N1 - Export Date: 14 December 2017 CODEN: PROTF Funding details: FCT, Fundação para a Ciência e a Tecnologia Funding details: UID/Multi/00612/2013, FCT, Fuel Cell Technologies Program Funding text: This work was supported by Fundação para a Ciência e a Tecnologia (FCT), Portugal, HS by project UID/Multi/00612/2013 and L.V.M by project PEst-OE/CTM/LA0024/2013 for INESC-MN and IN. 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PY - 2017
Y1 - 2017
N2 - Cilia are complex and dynamic organelles that have motility and sensory functions. Defects in cilia biogenesis and function are at the origin of human ciliopathies. In motile cilia, a basal body organizes the axoneme composed of nine microtubule doublets surrounding a central pair of singlet microtubules. The distal ends of axonemal microtubules are attached to the membrane by microtubule-capping structures. Little is known about the early steps of cilium assembly. Although cilia grow and resorb from their distal tips, it remains poorly understood where and when the components of the caps are first assembled. By using Atomic Force Microscopy in tapping mode, with resolution at the nanometer range and with minimum sample manipulation, we show that Tetrahymena cilia assembly requires transient assembly of structures, composed of three components that are placed asymmetrically on an early elongating axoneme. In small uncapped axonemes the microtubule central pair was never observed. Additionally, we show that cilia cap assembly is a multi-step process in which structures of different sizes and shapes are put together in close proximity before the axoneme appears capped. We propose that the cap modifies the axoneme microtubule rate of polymerization and present a model for Tetrahymena cilia cap assembly. © 2017 Elsevier GmbH
AB - Cilia are complex and dynamic organelles that have motility and sensory functions. Defects in cilia biogenesis and function are at the origin of human ciliopathies. In motile cilia, a basal body organizes the axoneme composed of nine microtubule doublets surrounding a central pair of singlet microtubules. The distal ends of axonemal microtubules are attached to the membrane by microtubule-capping structures. Little is known about the early steps of cilium assembly. Although cilia grow and resorb from their distal tips, it remains poorly understood where and when the components of the caps are first assembled. By using Atomic Force Microscopy in tapping mode, with resolution at the nanometer range and with minimum sample manipulation, we show that Tetrahymena cilia assembly requires transient assembly of structures, composed of three components that are placed asymmetrically on an early elongating axoneme. In small uncapped axonemes the microtubule central pair was never observed. Additionally, we show that cilia cap assembly is a multi-step process in which structures of different sizes and shapes are put together in close proximity before the axoneme appears capped. We propose that the cap modifies the axoneme microtubule rate of polymerization and present a model for Tetrahymena cilia cap assembly. © 2017 Elsevier GmbH
KW - AFM
KW - cap assembly
KW - cilia assembly
KW - re-ciliation.
KW - Tetrahymena
U2 - 10.1016/j.protis.2017.10.001
DO - 10.1016/j.protis.2017.10.001
M3 - Article
C2 - 29149699
VL - 168
SP - 697
EP - 717
JO - Protist
JF - Protist
SN - 1434-4610
IS - 6
ER -